rabbit anti dpp4  (Cusabio)

Journal: International Journal of Molecular Sciences

Article Title: Exploring the Link Between PACAP Signalling and Hyaluronic Acid Production in Melanoma Progression

doi: 10.3390/ijms262412049

Figure Lengend Snippet: mRNA ( A ) and protein ( B ) expression of PACAP receptors and DPP4 in human melanoma cells and in melanocytes. For RT-PCR and for Western blots, GAPDH and actin were used as internal controls, respectively. Images represent data from three independent experiments. Optical density of signals was measured, and the results were normalised to the optical density of control cultures. For panels ( A , B ), numbers below signals represent integrated densities of signals determined using ImageJ 1.40 g software. All data are the average of at least three different experiments. Statistical analysis was performed using Student’s t test. All data were normalised to GAPDH and actin. Data are expressed as the mean ± SD. Asterisks ( A , B ) indicate significant (* p < 0.05) alterations in the PACAP 1-38 treatment group as compared to the control. ( C ) Immunocytochemical reactions of DPP4 in melanoma and melanocytes. Magnification of the images is 60×. Scale bar: 5 µm.

Article Snippet: Following rinsing in PBS (pH 7.4), cells were permeabilised and blocked for 30 min at 37 °C in PBST (PBS containing 1% BSA and 0.1% Tween-20 [Amresco LLC., Solon, OH, USA]).The samples were incubated overnight at 4 °C with rabbit polyclonal anti-DPP4 antibody (Cell Signaling, Danvers, MA, USA) at 1:500 dilution, rabbit polyclonal anti-MITF antibody (Abcam, Cambridge, UK) at 1:500 dilution, rabbit polyclonal anti-RHAMM antibody (Novocastra, Newcastle, UK) at 1:400 dilution, rabbit polyclonal anti-HAS2 antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) at 1:200 dilution, and rabbit polyclonal anti-HAS3 antibody (Abcam, Cambridge, UK) at 1:500 dilution in PBST.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Software

Journal: International Journal of Molecular Sciences

Article Title: Exploring the Link Between PACAP Signalling and Hyaluronic Acid Production in Melanoma Progression

doi: 10.3390/ijms262412049

Figure Lengend Snippet: PACAP-driven signalling cascade regulating hyaluronan metabolism and melanocytic gene expression in melanoma. PACAP activates VPAC1 and PAC1 receptors on melanoma cells, triggering Gαs–adenylate cyclase signalling and increased cAMP production. Elevated cAMP activates PKA, which subsequently enhances phosphorylation of downstream transcription factors including CREB and Sox9, while modulating Sox10 and TYRP expression. PACAP signalling also upregulates DCT and MITF, key regulators of melanocyte differentiation. On the extracellular side, PACAP stimulation increases the expression of hyaluronan synthases (HAS2–3) and hyaluronidases (Hyal2, Hyal4), leading to elevated hyaluronan (HA) synthesis and turnover. HA interacts with receptors such as RHAMM and CD44, influencing extracellular matrix remodelling and cell signalling. Integrins (αV and β3) and DPP4 are shown as additional surface components affected by PACAP-mediated pathways. Red arrows indicate upregulated molecules or processes, while blue arrows represent downregulation, while green dots indicate no alterations in protein expression of these proteins.

Article Snippet: Following rinsing in PBS (pH 7.4), cells were permeabilised and blocked for 30 min at 37 °C in PBST (PBS containing 1% BSA and 0.1% Tween-20 [Amresco LLC., Solon, OH, USA]).The samples were incubated overnight at 4 °C with rabbit polyclonal anti-DPP4 antibody (Cell Signaling, Danvers, MA, USA) at 1:500 dilution, rabbit polyclonal anti-MITF antibody (Abcam, Cambridge, UK) at 1:500 dilution, rabbit polyclonal anti-RHAMM antibody (Novocastra, Newcastle, UK) at 1:400 dilution, rabbit polyclonal anti-HAS2 antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) at 1:200 dilution, and rabbit polyclonal anti-HAS3 antibody (Abcam, Cambridge, UK) at 1:500 dilution in PBST.

Techniques: Gene Expression, Phospho-proteomics, Expressing

Journal: Scientific Reports

Article Title: Establishment of well-differentiated camelid airway cultures to study Middle East respiratory syndrome coronavirus

doi: 10.1038/s41598-022-13777-y

Figure Lengend Snippet: Establishment and characterization of Bactrian camel and llama AEC cultures. ( A ) Immunofluorescence analysis showing the development of tight-junctions (ZO-1, white) and ciliogenesis (β-tubulin, red) in Bactrian camel and llama AEC cultures over time from 1-day to 4 weeks post ALI exposure. The cells were counterstained with DAPI (blue) to visualize the nuclei. ( B,C ) Ciliogenesis quantification of camel and llama AEC cultures overtime, respectively. Ciliation was quantified by measuring the area above a fluorescence intensity threshold of five random images acquired per condition. ( D ) Transepithelial electrical resistance (TEER) measurement of camel and llama AEC cultures overtime during the differentiation. ( E ) Epithelial morphology of ex vivo tissues (upper panel) and well-differentiated camel and llama AEC cultures (lower panel). ( F ) DPP4 expression in well-differentiated camel and llama AEC cultures, with Vero cells as a positive control. Scale bar is 20 µm.

Article Snippet: To visualize the cellular receptor DPP4, a rabbit polyclonal antibody against DPP4 (Abcam, ab28340) was used.

Techniques: Immunofluorescence, Fluorescence, Ex Vivo, Expressing, Positive Control

Journal: bioRxiv

Article Title: Establishment of well-differentiated camelid airway cultures to study Middle East respiratory syndrome coronavirus

doi: 10.1101/2021.11.10.468038

Figure Lengend Snippet: ( A ) Immunofluorescence analysis showing the development of tight-junctions (ZO-1, white) and ciliogenesis (β-tubulin, red) in Bactrian camel and llama AEC cultures over time from 1-day to 4 weeks post ALI exposure. The cells were counterstained with DAPI (blue) to visualize the nuclei. ( B ) and ( C ) Ciliogenesis quantification of camel and llama AEC cultures overtime, respectively. Ciliation was quantified by measuring the area above a fluorescence intensity threshold of five random images acquired per condition. ( D ) Transepithelial electrical resistance (TEER) measurement of camel and llama AEC cultures overtime during the differentiation. ( E ) Epithelial morphology of ex vivo tissues (upper panel) and well-differentiated camel and llama AEC cultures (lower panel). ( F ) DPP4 expressions of well differentiated camel and llama AEC cultures, with Vero cells as a positive control. Scale bar is 20 μm.

Article Snippet: To visualize the cellular receptor DPP4, a rabbit polyclonal antibody against DPP4 (Abcam, ab28340) was used.

Techniques: Immunofluorescence, Fluorescence, Ex Vivo, Positive Control